Digital
Imaging
GS-800
Coomassie
or silver stained gels can be digitally imaged using a
GS-800 calibrated densitometer.
Fx
ProPlus
Gels
stained with popular fluorescent stains such as Sypro
Ruby, Deep Purple or Flamingo for total protein content
or specific protein subsets such as oligohistidine tagged
proteins, glycoproteins and phosphoproteins using ProQ
range of stains can all be imaged with the FXProPlus laser
scanner.
The
FXProPlus can also be use in conjunction with a range
probes for 'multiplexed western blots' or for enhanced
chemifluorescense (ECF) as well as detection of radioactive
isotopes inconjunction with both B and K-type phosphor
imaging screens.
DIGE
Analysis
Differential
In Gel Electrophoresis (DIGE) analysis involved the labelling
of samples with size and charged matched fluorescent dyes
and then running up to three samples on one gel. This
allows experiments to be designed with an internal standard
- a sample made up of a fraction of all samples being
processed in that analysis set. The internal standard
is run on every gel. The effect of this is to greatly
reduce gel-gel variation, decrease the analysis time and
enhance accuracy the statistical analysis of a set of
samples.
Comparison
of two cancer cell line using DIGE. Image courtesy of
Dr B. Crossett
