Peptide
Mass Fingerprinting
The
simplest and quickest method of identifying a protein
by mass spectrometry is by peptide mass fingerprinting
(PMF). The sample is digested with a protease such as
trypsin and the resulting proteolytic peptides analysed
by MS. The masses of the peptides obtained are used to
interrogate a database of known proteins and the identity
of the protein is determined.
Samples
are processed using the QSTAR XL mass spectrometer running
with the oMALDI ionisation source or with the Voyager
DE STR.
Registered
users can book the QSTAR by clicking here,
and book the Voyager here.
