De
novo Sequencing
MALDI
MS/MS
Once
a MALDI-MS spectra has been obtained an individual peptide
ion can be selected for further fragmentation. Interpretation
of the resultant product, or daughter ions, can be used
to determine the peptide sequence and identify post-translational
modifications.
The
results from MS and MS/MS analysis can be combined and
searched against databases of known proteins to identify
those present in the sample. MS/MS data has the advantage
over PMF in that it a higher level of confidence in the
identity of the proteins can be attained and it is better
able to identify multiple proteins within the same sample.
The sequence
data alone can also be used to BLAST search sequence database
to try and identify proteins which are not in the sequence
database, such as those from species which have not had
their genome sequenced.
Registered
users can book the QSTAR by clicking here.
Uses
interested in LC-ESI-MS and LC-MADLI-MS should contact
the Facility
Manager
for further details.
