2D Fluorescence
Difference Gel Electrophoresis (DIGE) Workshop
Aims:
The focus of this workshop is the theory and practical
application of 2D Fluorescence Difference Gel Electrophoresis
(DIGE) for quantitative proteomic analysis. The lecture
components will cover the principles of the technique,
the correct experimental design and data analysis, the
practical component will enable participants to scan pre-run
gels and perform their own data analysis.
Sessions:
The course will include lectures and practical sessions
on:
Who is
this course for?
This one-day workshop is aimed at the general research
community interested in picking up this valuable technique.
A sound knowledge of 2D gel based proteomics is assumed,
so it is suited to people who have just completed the
2D Gel Electrophoresis Course immediately prior to this
workshop.
Invited
contributors:
TBC.
Is there
any practical work?
Yes, participants will perform there own DIGE labelling
reaction (test samples provided), and analysis the results
by 1D SDS-PAGE, thus gaining experience in multiplex scanning
using the Typhoon Trio. A previously prepared DIGE dataset
will be used during the practical data analysis session.
What
else do I get?
Full set of course materials. Lunch and refreshments
are provided..
When,
where, how?
The next course will be run Friday 29th September, at
the APAF facility in Macquarie University.
Early bird
registration (minimum of 1 month prior to the course)
is $500 (academic)/$600 (industrial). If you register
less than a month before the course it costs an addition
$100.
For registration
please contact the Andrew
Gilbert at the Macquarie Node, and other enquiries
contact the workshop coordinator: Dr Ben
Crossett.
