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2D Gel Electrophoresis


Isoelectric Focusing
The facility is equipped to run 7, 11, 17cm or 24cm 2D gels. The first dimension, using a range of Immobilised pH gradients, are focused using one of four BioRad IEF cell or a Pharmacia Multipohore. Registered users can book a session by clicking on the link to the appropriate calendar:

IEF1 IEF2 IEF3 IEF4 Multiphore

SDS-PAGE
A wide variety of SDS-PAGE gels, including gradient gels, can be run using the mini-protean, criterion protean II XL and Dodeca cell tanks.

With the four Protean II XL tanks and the Dodeca Cell registered users can book then by clicking on the appropriate link:

Tank 1 Tank 2 Tank 3 Tank 4 Dodeca

Gel Blotting
The facility has a semi-dry blotter, a criterion blotter and Trans-Blot cell.

 

   

 



© 2005 Biomedical Node, Australian Proteome Analysis Facility, The University of Sydney, NSW 2006

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